Full Time Academic-Projec Bicentenario. Universidad de Santiago de Chile.
PhD in Chemistry, University of Santiago, Chile, 2008. Bachellor in chemistry (Químico y Licenciado en Química). University of Santiago, Chile, 2003.
We proposes an innovative strategy for the fabrication of a biosensor for the electrochemical detection of neurotransmitters, using nanoelectrode arrangements based on thin films nanostructures and functionalized with copper phthalocyanines. The functionalization with copper catalytic complex is based on the fact that they have shown high selectivity for the oxidation of phenols and also for molecules of catechol family, such as the neurotransmitters, dopamine (DOPA) and epinephrine (EP).
The construction and characterization of nanosensor arrangements based on hybrid self assembled monolayers of dodecanothiol/mercaptoundecanoic acid and hexanothiol/mercaptoundecanoic acid is also formulated. These monolayers will be functionalized with copper phthalocyanines. These arrangements of catalysts will be characterized using electrochemical techniques. The morphological and chemical characterization will be carried out using scanning tunneling microscopy (STM) and photoelectron X-ray spectroscopy (XPS). The sensitivity, speed of response and selectivity of the nanosensor for the oxidation of dopamine and ascorbic acid will be evaluated.
Porous alumina "templates" will be used for creating the arrangements on the surface and compare the answer with arrangements made up of mixtures of thiols with different functionalities. The electrochemical characterization of the arrangements will be carried out using cyclic voltammetry and their morphology will be characterized by using STM and AFM. We will evaluate the formation of arrangements starting from thiols mixtures in solution and these arrangements will be functionalized with the copper phthalocyanines. The density of redox states (DOS) will be determined by STM, establishing dependences among DOS with different architectures and the different electrocatalytic responses observed.
The purpose of this work is to develop a method for the production of arrangements of nanoelectrode functionalized with copper phthalocyanines. The nanoelectrode arrangement will be used for the electrochemical detection of two neurotransmitters in the presence of interfering agents. We will determine the classic parameters for the performance of a sensor, i.e. selectivity, detection limit and time of response of the nanoelectrode arrangement study with this way the advantages of the nanosensor device. It is expected that the functionalization with the copper phthalocyanines will improve the selectivity of the nanosensor arrangement substantially, and this will be evaluated with regard to the information reported in literature and from the performance of the system without functionalization.
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